abstract：:Prostaglandin E2 (PGE2) stimulated cAMP production in the MOB 3-4-F2 cell line, a subclone of the osteoblast-like MOB 3-4 cell line. After being cultured in alpha-minimum essential medium supplemented with 10% heat-inactivated foetal calf serum (HIFCS), cells responded to PGE2 (greater than or equal to 50 ng/ml) with ...

journal_title：Cellular signalling

authors： Kawase T,Orikasa M,Suzuki A

更新日期：1991-01-01 00:00:00

abstract：:The effects of ouabain and monensin upon basal and carbachol-stimulated inositol phospholipid breakdown in rat cerebral cortical miniprisms have been investigated. Basal inositol phospholipid breakdown was increased by both compounds at both 6 and 18 mM K+. Enhancement of the carbachol response at 6 mM, but not at 18 ...

journal_title：Cellular signalling

authors： Tiger G,Fowler CJ

更新日期：1991-01-01 00:00:00

abstract：:Human neutrophils pre-incubated with granulocyte-macrophage-colony-stimulating factor (GM-CSF) exhibit an enhanced mobilization of calcium in response to secondary stimuli such as chemotactic factors. The mechanisms underlying this priming effect of GM-CSF were examined. It was first demonstrated that the additional c...

journal_title：Cellular signalling

authors： Naccache PH,Hamelin B,Gaudry M,Bourgoin S

更新日期：1991-01-01 00:00:00

abstract：:The implication of protein kinase C in the phenomenon of pancreatic acinar cell desensitization to carbamylcholine, caerulein and the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) was investigated using a potent PKC inhibitor, staurosporine. At a concentration of 1 microM, staurosporine caused a maximum 64%...

journal_title：Cellular signalling

authors： Morisset J,Paquette B,Benrezzak O

更新日期：1991-01-01 00:00:00

abstract：:High affinity Ins(1,4,5)P3-binding sites of permeabilized hepatocytes are probably the ligand recognition sites of the receptors that mediate the effects of Ins(1,4,5)P3 on intracellular Ca2+ mobilization. We have now solubilized these sites from rat liver membranes in the zwitterionic detergent, CHAPS, and shown that...

journal_title：Cellular signalling

authors： Bond JM,Taylor CW

更新日期：1991-01-01 00:00:00

abstract：:The induction of cytolytic activity in PC60, a murine T-cell hybridoma, is paralleled by a rise in the level of BLT-esterase (N-alpha-benzyloxycarbonyl-L-lysine thiobenzyl esterase), a serine esterase specific for activated T-cells. Both interleukin-1 (IL-1) and dibutyryl cAMP were albe to increase the esterase activi...

journal_title：Cellular signalling

authors： Schlegel-Haueter SE,Aebischer F

更新日期：1990-01-01 00:00:00

abstract：:DMSO differentiated U937 cells responded to 10(-6) M LTD4, LTB4 and FMLP with an increase in both InsP formation and [Ca2+]i. FMLP caused a greater rise in InsPs than either LTD4 or LTB4, which were equivalent. LTD4, however, caused a greater increase in [Ca2+]i than LTB4 (4-fold) or FMLP. The FMLP [Ca2+]i and InsP re...

journal_title：Cellular signalling

authors： Pollock K,Creba J

更新日期：1990-01-01 00:00:00

abstract：:The effects of tamoxifen (TAM) on cell proliferation and voltage-dependent K+ channels were studied on the mouse neuroblastoma cells NG 108-15. TAM inhibited cell proliferation with an effective dose inducing a half maximum effect (ED50) of 2 microM and was cytotoxic from and beyond 2.5 microM. TAM accelerated the app...

journal_title：Cellular signalling

authors： Rouzaire-Dubois B,Dubois JM

更新日期：1990-01-01 00:00:00

abstract：:Inositol 1,4,5-trisphosphate 5-phosphatase catalyses the dephosphorylation of the phosphate in the 5-position from inositol 1,4,5-trisphosphate and inositol 1,3,4,5-tetrakisphosphate. One particulate and two soluble enzymes were previously described in bovine brain. In this study, we have obtained a precipitating anti...

journal_title：Cellular signalling

authors： Verjans B,Hollande F,Moreau C,Lejeune C,Erneux C

更新日期：1990-01-01 00:00:00

abstract：:Whole-cell currents activated by bath applications of acetylcholine (ACh) (10-30 microM) were recorded from patch-clamped myotubes of the mouse C2 cell line. Increasing concentrations of forskolin caused a dose-dependent fast decay of ACh-activated currents as compared to the long-lasting ACh-currents in control cells...

journal_title：Cellular signalling

authors： Giovannelli A,Farini D,Gauzzi MC,Alema S,Eusebi F

更新日期：1990-01-01 00:00:00

abstract：:Exogenous tritiated phosphatidylinositol bisphosphate added to antennal preparations from locust and cockroach was hydrolysed releasing inositol trisphosphate. High activity of phospholipase C was detected in the soluble as well as in the membrane fraction. At low free calcium concentrations hydrolysis of the labelled...

journal_title：Cellular signalling

authors： Boekhoff I,Strotmann J,Raming K,Tareilus E,Breer H

更新日期：1990-01-01 00:00:00

abstract：:The present study was undertaken to investigate whether purified ras proteins can affect the activity of polyphosphoinositide specific phospholipase C in a cell-free membrane system. For this purpose we used homogenous preparations of the proto-oncogenic (H-ras(gly 12)) and the oncogenic (H-ras(val 12)) forms of the h...

journal_title：Cellular signalling

authors： Cockcroft S,Bar-Sagi D

更新日期：1990-01-01 00:00:00

abstract：:Cortical and medullary thymocytes can be separated from each other by virtue of the fact that only cortical thymocytes bear peanut agglutinin (PNA) receptors. The mitogenic responses of subpopulations of thymocytes were studied. We have confirmed the results of Conlon et al. [(1982) J. Immun. 128, 797-801], that lecti...

journal_title：Cellular signalling

authors： Zilberman Y

更新日期：1990-01-01 00:00:00

abstract：:The present study has examined the effects of adenosine A1 receptors on second messenger processes in GH3 cells. A1 receptors are present which are shown to inhibit adenylate cyclase in a GTP-requiring manner. Hormone (VIP) stimulation is also absolutely required for the observation of inhibition. Adenosine A1 recepto...

journal_title：Cellular signalling

authors： Cooper DM,Caldwell KK,Boyajian CL,Petcoff DW,Schlegel W

更新日期：1989-01-01 00:00:00

abstract：:Staphylococcal alpha-toxin at subcytotoxic concentrations stimulated phosphatidylinositol turnover and arachidonic acid release in undifferentiated cultures of pheochromocytoma PC12 cells. Stimulation of phospholipase A2 but not C was dependent on extracellular calcium. Addition of staphylococcal alpha-toxin to PC12 c...

journal_title：Cellular signalling

authors： Fink D,Contreras ML,Lelkes PI,Lazarovici P

更新日期：1989-01-01 00:00:00

abstract：:Hepatocyte membranes from both lean and obese Zucker rats exhibited adenylate cyclase activity that could be stimulated by glucagon, forskolin, NaF and elevated concentrations of p[NH]ppG. In membranes from lean animals, functional Gi was detected by the ability of low concentrations of p[NH]ppG to inhibit forskolin-a...

journal_title：Cellular signalling

authors： Houslay MD,Gawler DJ,Milligan G,Wilson A

更新日期：1989-01-01 00:00:00

abstract：:Exposure of brown fat cells to phenylephrine, an agonist of alpha-1 adrenergic receptors, activates a phospholipase A2 which releases arachidonic acid. Since receptor activation of phospholipase A2 requires calcium, experiments were undertaken to define more precisely the role played by calcium in the regulation of en...

journal_title：Cellular signalling

authors： Schimmel RJ

更新日期：1989-01-01 00:00:00